منابع مشابه
Update on Recombinational Cloning with Plant Gateway Vectors Recombinational Cloning with Plant Gateway Vectors
The study of biological systems relies to a large extent on DNA cloning technologies enabling the analysis of recombinant genes through transgenic research. In this context, the advent of recombinational cloning methods was a significant progress because DNA fragments can now be assembled regardless of their sequence. In particular, the Gateway system was designed to join fragments in a predefi...
متن کاملGateway-compatible vectors for plant functional genomics and proteomics.
Gateway cloning technology facilitates high-throughput cloning of target sequences by making use of the bacteriophage lambda site-specific recombination system. Target sequences are first captured in a commercially available "entry vector" and are then recombined into various "destination vectors" for expression in different experimental organisms. Gateway technology has been embraced by a numb...
متن کاملTMV-Gate vectors: Gateway compatible tobacco mosaic virus based expression vectors for functional analysis of proteins
Plant viral expression vectors are advantageous for high-throughput functional characterization studies of genes due to their capability for rapid, high-level transient expression of proteins. We have constructed a series of tobacco mosaic virus (TMV) based vectors that are compatible with Gateway technology to enable rapid assembly of expression constructs and exploitation of ORFeome collectio...
متن کاملGateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction
Yuji Tanaka1, Tetsuya Kimura2, Kazumi Hikino3, Shino Goto3,4, Mikio Nishimura3,4, Shoji Mano3,4 and Tsuyoshi Nakagawa1 1Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 2Department of Sustainable Resource Science, Graduate School of Bioresources, Mie University, 3Department of Cell Biology, National Institute for Basic Biology, 4Dep...
متن کاملImproved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants.
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2018
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot094920